Pipeline job
demo_phage
DemoPhage synthetic mini sequence for Phase 0 harness testing
succeeded
Length228 bp
GC56.58%
Targetphage
Baseline ORFs2
Exports
Draft review queue
2 gene callsDraft OK0
Check start0
Check boundary0
Needs evidence2
Phase 2 derives this queue from ORF length, start codons, and neighbor gaps/overlaps. BLAST/Pfam/tRNA evidence is still pending, so these are review prompts rather than final annotation calls.
tRNA evidence
AragornNo tRNAs were detected in this genome.
Review warnings
- Sequence is 228 bp, which is much shorter than a typical complete phage genome.
- Baseline ORFs are not final gene calls.
- Compare against Glimmer/GeneMark/PHEONA and instructor review before accepting annotations.
Tool availability
blastnmissing
blastpmissing
aragorninstalled
hmmscanmissing
genemarkmissing
glimmer3missing
makeblastdbmissing
trnascan_semissing
Baseline ORFs
six-frame start/stop ORF scan| ID | Strand | Frame | Start | End | Codons | Length |
|---|---|---|---|---|---|---|
| +1.1 | + | 1 | 10 | 114 | ATG → TAA | 34 aa |
| +1.2 | + | 1 | 124 | 228 | ATG → TAG | 34 aa |
Review details
Phase 2 draft curation| Gene | ORF | Status | Coordinates | Flags | Decision | Note | Save |
|---|---|---|---|---|---|---|---|
| gene-001 | +1.1ATG → TAA, 34 aa | needs evidence | 10..114 (+1)adjacent/first | Short ORF (34 aa) | |||
| gene-002 | +1.2ATG → TAG, 34 aa | needs evidence | 124..228 (+1)9 nt gap | Short ORF (34 aa) |